Laboratory Technician II, Sequencing Technologies

Day Zero Diagnostics - Boston, MA

Full-time - Entry Level

Boston, MA

Computer and Electronic Product Manufacturing

About the position

Day Zero Diagnostics (DZD) is seeking a Laboratory Technician II to support the Sequencing Technologies team. This role involves high-throughput sample processing, DNA library preparation, quality control assessments, and whole-genome sequencing on both short- and long-read platforms. The technician will work in a state-of-the-art facility, contributing to the mission of modernizing infectious disease diagnostics and treatment.

Responsibilities

• Perform DNA library preparation, QC assessments including TapeStation and fluorescent quantitation assays, and WGS for diagnostic workflows on short-read and long-read sequencing platforms with minimal guidance.
• Execute repetitive NGS workflows and strictly follow SOPs for specimen handling, processing, and reporting.
• Collaborate with interdisciplinary teams and integrate NGS workflows into broader research initiatives.
• Identify risks/issues that may adversely affect test performance and notify the appropriate supervisor.
• Maintain meticulous documentation on sample processing and tracking, QC checkpoints, and any corrective actions taken when tests deviate from the laboratory's established SOPs.
• Train/mentor wet-lab team members, provide guidance on SOPs, techniques and workflow improvements.
• Conduct routine maintenance of instruments, manage inventory, and order essential reagents and consumables.
• Prepare and communicate SOPs, data summaries, technical reports, and presentations for internal use.

Requirements

• A bachelor's degree in a chemical, physical, biological, or clinical laboratory science from an accredited institution, or a 4 year medical or biotechnology degree, is required to fulfill CLIA requirements.
• 1-2 years of wet lab experience in academic and/or industry setting.
• Experience with laboratory methods/techniques associated with NGS pipelines, including Illumina-based DNA library construction, QC, and sequencing.
• Strong organizational skills with the ability to handle multiple projects and high volumes of sample processing.
• Properly use and maintain standard laboratory equipment.

Nice-to-haves

• Experience with long-read sequencing technologies, including ONT, is highly desirable.
• Proficiency in nucleic acids extraction, quantitation, gel electrophoresis, PCRs, and related techniques is desired.
• Experience with liquid handling and automated NGS pipelines is desired.
• Clinical laboratory testing experience is desired.

Benefits

• Opportunities for skill development and training in a multidisciplinary environment.
• Engagement with emerging technologies and a supportive team atmosphere.