Plant Tissue Culture Technicians play a crucial role in laboratory environments where they utilize aseptic (sterile) techniques to propagate plants. This position requires a minimum of an Associate's degree in biotechnology or a related field, with more advanced roles necessitating a Bachelor's degree. Technicians are responsible for taking clippings from plants and growing them on agar media in sterile containers, a process known as micropropagation. This method allows for the mass production of certain plant types while preventing the transmission of diseases. Additionally, it enables the production of clones, which helps maintain the genetic characteristics of the plants by avoiding pollination. The work is typically performed in a laminar flow hood to prevent contamination from bacteria or fungi. In some instances, technicians may also grow plant cells in culture using a bioreactor. It is essential for technicians to be aware of and comply with industry regulations and certification requirements, as many plants and plant products are regulated. A strong foundation in biotechnology-related skills and knowledge is vital, as technicians will be involved in planting, maintaining, and evaluating plants for disease resistance and other traits. Technicians are expected to follow Standard Operating Procedures (SOPs), maintain detailed records, and collect data. Proficiency in data management, including the use of spreadsheets and cloud-based electronic notebook software, is often required. Responsibilities also include maintaining an inventory of chemicals, media, and stock solutions, as well as safely disposing of chemical and biological waste. Technicians may need to order supplies and reagents necessary for experiments and plant growth, keeping meticulous records of all orders. In addition to technical skills, effective communication and teamwork are essential, as technicians often work as part of a team. A positive attitude and the ability to communicate effectively with team members are desirable traits. Those with advanced degrees may be tasked with designing experiments and research projects and supervising others. Experience with greenhouses and indoor growing operations is beneficial, as is familiarity with horticulture, plant biology, and genetics. Technicians should also be able to recognize signs of plant disease and identify important traits.