MD Anderson Cancer Center - Houston, TX
posted 5 months ago
The Research Assistant I position at MD Anderson Cancer Center is a full-time role focused on supporting leukemia research through various laboratory techniques. The primary responsibilities include maintaining and establishing tissue culture cell lines, which necessitates a strong understanding of sterile techniques and the ability to observe and identify cell changes under a microscope. The role requires the preparation of sterile media and solutions, monitoring tissue culture supplies, and ensuring efficient laboratory operations. Additionally, the assistant will prepare cells for cryogenic storage and reestablishment using small vials, dry ice, and liquid nitrogen. In terms of molecular techniques, the Research Assistant will isolate, purify, and analyze RNA, DNA, and proteins. This includes performing hybridization, gel electrophoresis, autoradiography, ECL staining, PCR reactions, quantitative RT-PCR assays, DNA sequencing, and assays for signal transducing enzymes. A basic knowledge of solution and buffer preparation, particularly regarding ionic concentration and pH, is essential. The candidate should also have training in the use and safety of radioisotopes. The position also involves routine maintenance of laboratory equipment, including ultra-low freezers, liquid nitrogen, and CO2 levels. The assistant will be responsible for maintaining high-speed centrifuges, pH meters, and microfuges, and must be able to manually operate and maintain lab equipment. Compliance with institutional safety policies, including Universal Precautions and OSHA standards, is mandatory when handling hazardous materials. The role may require the use of protective garments and equipment, as well as working in a fume hood or biological safety cabinet. Other duties may be assigned as needed.