Alloy Therapeutics - Waltham, MA

posted 5 months ago

Full-time - Entry Level
Remote - Waltham, MA
Professional, Scientific, and Technical Services

About the position

The Research Associate position at Alloy Therapeutics is a full-time role within the Translational Research team, which plays a crucial part in supporting drug discovery partners through in vitro and in vivo testing of therapeutic drug candidates. The team is dedicated to providing robust functional characterization of biologics, including monoclonal antibodies, multispecific antibodies, TCR-mimic antibodies, chimeric antigen receptor (CAR)-expressing cell therapies, and genetic medicines. As a Research Associate, you will be responsible for running validated assay systems to test therapeutic biologics, including antibodies and cell therapies, utilizing state-of-the-art high-throughput methods to evaluate the activity of novel biologics on human cells in disease-specific assay systems. In this role, you will also provide operational support to the Translational Research team, which includes tasks such as cell line maintenance, reagent generation and preparation, and inventory management. The position requires a mindset that embraces continuous learning and the ability to manage multiple independent projects simultaneously. You will report to a Senior Scientist and will have the autonomy to own various stakeholders, projects, and tasks in a dynamic start-up environment, contributing to the fast-paced culture at Alloy Therapeutics. The ideal candidate will have relevant experience in immunology, cell biology, molecular biology, or a closely related field, and will be expected to create functional data characterizing the disease-modulating activity of novel biologic therapeutics, investigate novel therapies, assess comparative activity of biologics, coordinate with team members, and communicate research results effectively.

Responsibilities

  • Run validated assay systems for testing therapeutic biologics, including antibodies and cell therapies.
  • Utilize high-throughput methods to evaluate the activity of novel biologics on human cells in disease-specific assay systems.
  • Provide operational support to the Translational Research team through cell line maintenance, reagent generation, and inventory management.
  • Create functional data characterizing the disease-modulating activity of novel biologic therapeutics.
  • Investigate novel antibody- and cell-based therapies for various clinical indications.
  • Assess comparative activity of novel biologics to research-grade control reagents or clinical-stage assets.
  • Coordinate between Translational Research team members and the internal Alloy scientific team to ensure high-quality data delivery.
  • Communicate research results to the Translational Research team, the broader Alloy scientific team, and external research partners.

Requirements

  • Bachelor's degree in Immunology, Cell Biology, Molecular Biology, or a related scientific field.
  • Hands-on experience with cell biology techniques, including culture of mammalian primary cells and immortalized cell lines.
  • Experience with immunoassays such as Western blots, ELISAs, and plate-based colorimetric/fluorescent reporter assays.
  • Experience designing and executing experiments utilizing multiparameter flow cytometry.
  • Experience with cell line engineering and validation (e.g., stable retroviral transduction, transient DNA/mRNA transfection, CRISPR).
  • Experience with cytokine release assays using primary human cells.
  • Experience with antibody-dependent cellular cytotoxicity and phagocytosis using primary human cells.
  • Experience with T cell cytotoxicity assays using endpoint flow cytometry measurements and kinetic live-cell imaging.
  • Experience with primary human immune cell activation assays using multparameter flow cytometry and ELISpot imaging techniques.
  • Experience with optimization of biological activity assays relying on fluorometric or colorimetric readout.
  • Experience with digital ELN/LIMS systems.
  • Exemplary oral and written communication skills.
  • Desire to learn new skills and techniques in immunology.
  • Ability to prioritize and advance goals while working on multiple projects simultaneously.

Nice-to-haves

  • Master's degree in a related scientific field.
  • Hands-on experience working with mammalian cells in a BSL2+ laboratory environment.
  • Ability to work across various US and international time zones.
  • Willingness to accommodate occasional travel as needed (10%).

Benefits

  • Continuing education credits
  • Paid parental leave
  • Disability insurance
  • Health insurance
  • Unlimited paid time off
  • Dental insurance
  • Paid time off
  • Work from home
  • Parental leave
  • Employee assistance program
  • Vision insurance
  • 401(k) matching
  • Life insurance
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